J Cell Biol 19 February 2001 152 (4) 683 evidence that this is achieved by coupling of the mitochondrial outer and inner membranes by the mitochondrial fusion machinery Fzo1 the first known mediator of mitochondrial fusion spans the outer membrane twice exposing a short loop to the intermembrane space and 216 μl from the bottom Dietary Feeding of Dibenzoylmethane Inhibits Prostate Cancer in Transgenic Adenocarcinoma of the Mouse Prostate Model The cell pellet was then resuspended in 500 μL PBS and the resulting cell suspension was passed through a 26 5-gauge needle thrice Mills PK Beeson WL Phillips RL Fraser GE Cohort study of diet lifestyle and

Inhibition of HIV

Oct 13 2009Eukaryotic translation initiation factor eIF5A has been implicated in HIV-1 replication This protein contains the apparently unique amino acid hypusine that is formed by the post-translational modification of a lysine residue catalyzed by deoxyhypusine synthase and deoxyhypusine hydroxylase (DOHH) DOHH activity is inhibited by two clinically used drugs the topical fungicide ciclopirox and

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Several new classes of antiviral agents targeting viral replication mechanisms or cellular proteins/processes are under development This review gives an overview of novel strategies targeting the virus and/or the host cell for counteracting influenza virus infection

Jul 27 2006Introduction Ubiquitination is a post‐translational modification in which a small conserved peptide ubiquitin is appended to target proteins in the cell through a series of complex enzymatic reactions (Hershko and Ciechanover 1992) The ubiquitin–proteasome system plays an important role in controlling the levels of various cellular proteins and regulates basic cellular processes such

Nuclear export of proteins The role of nuclear retention

A quantitative comparison of the export characteristics of NLS-PK and NLS-PK-RNP/GR (Figure 6C) illustrated that the presence of the RNP and GR do- mains of nucleolin strongly inhibited nuclear export of NLS-PK while 450/0 of NLS-PK had reached the cytoplasm 8 hr after nuclear injection only 10% of the NLS-PK-RNP/ GR protein had been able to

In most human somatic tissues these specialized nucleoprotein complexes are challenged after each round of DNA replication From yeast to humans replicative loss of telomeric DNA is replenished by the action of the RNP enzyme telomerase or by the telomerase-independent alternative lengthening of telomeres (ALT)

The generation of the amyloid-β peptide (Aβ) through the proteolytic processing of the amyloid precursor protein (APP) is a central event in the pathogenesis of Alzheimer's disease (AD) Recent studies highlight APP endocytosis and localization to lipid rafts as important events favoring amyloidogenic processing However the precise mechanisms underlying these events are poorly

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Lynch MA Long-Term Potentiation and Memory Physiol Rev 84 87–136 2004 10 1152/physrev 00014 2003 —One of the most significant challenges in neuroscience is to identify the cellular and molecular processes that underlie learning and memory formation The past decade has seen remarkable progress in understanding changes that accompany certain forms of acquisition and

Jul 01 2013In the plant kingdom SAM works as a primary biological methyl (CH 3) donor notably required for polyamine production and for the regulation of a number of essential cellular processes such as cell division synthesis of chlorophyll and maintenance of integrity of cell walls and membranes 20 The synthesis of Met is tightly regulated

a tiny pellet filled with ricin toxin into the subcutaneous tissue of his leg He died several days later On autopsy the tiny pellet was found and de- The shape of the bacterial cell is determined by the rigid cell wall The interior of the cell contains the nuclear material (DNA) cytoplasm and cell

Massachusetts General Hospital on Instagram

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Sep 27 2010Regulation of eukaryotic cytochrome oxidase assembly occurs at the level of Cox1 translation its central mitochondria-encoded subunit Translation of COX1 messenger RNA is coupled to complex assembly in a negative feedback loop the translational activator Mss51 is thought to be sequestered to assembly intermediates rendering it incompetent to promote translation

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Oct 15 2019Nematodes were collected in M9 buffer with two washes to remove excess bacteria Nematode pellet was obtained by centrifugation at 4C snap-frozen in liquid nitrogen and stored in −80C until homogenization was performed Nematode pellet was homogenized in 50% acetonitrile 0 3% formic acid

Jul 01 2013In the plant kingdom SAM works as a primary biological methyl (CH 3) donor notably required for polyamine production and for the regulation of a number of essential cellular processes such as cell division synthesis of chlorophyll and maintenance of integrity of cell walls and membranes 20 The synthesis of Met is tightly regulated

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